Nathan P. Coussens, Ph.D., is a senior research scientist in the Division of Pre-Clinical Innovation at the National Center for Advancing Translational Sciences (NCATS), where he applies his diverse scientific background to design and optimize biologically relevant biochemical and cell-based assays for high-throughput screening. He works with a highly collaborative and multidisciplinary team to develop small molecule probes relevant to a variety of human diseases. Dr. Coussens also serves as editor-in-chief of the Assay Guidance Manual, a growing online eBook of best practices for preclinical assay development and implementation that has become the go-to resource for scientists in industry and academia. Prior to joining NCATS in 2013, Dr. Coussens was a postdoctoral fellow at the National Cancer Institute (NCI), where he combined biophysical studies, cell biology and high-resolution imaging to interrogate signaling events initiated by the T-cell antigen receptor. He received his Ph.D. in biochemistry from the University of Iowa, where he applied structural and biophysical approaches to study host-pathogen interactions and, as a postdoctoral fellow of the Interdisciplinary Immunology Program there, utilized crystallographic and thermodynamic studies to inform the development of small molecules that target a host evasion mechanism exploited by pathogenic bacteria.